RIP sebagai antikanker

December 4th, 2008

ABSTRAK

            Ribosome-inactivating protein (RIP) merupakan protein yang umumnya berasal dari tanaman dan bersifak sitotoksik terhadap sel mamalia. Hal ini  disebabkan oleh aktivitas rRNA N-glikosidae dan purin glikosidase sehingga sel mati. Selain itu berbagai RIP juga memacu apoptosis bagi sel yang rusak, dan sebagai antioksidan sehingga RIP merupakan kandidat antikanker yang baik. RIP dapat dikonjugasikan dengan antibodi monoklonal sebagai imunotoksin sehingga efeknya sangat selekif hanya pada sel target. 

 

Kata kunci: Ribosome-inactivating protein (RIP), antikanker, imunotoksin

 

CV

December 4th, 2008

CURICULUM VITAE

 

01. Name                     :  Sudjadi, Pharm., MSc., PhD.

   Professor of Molecular Biology

02. Sex                        : Male

03. Place/date of birth : Yogyakarta, October 30th,1947

04. Home address        : Depokan KG II 201C, Kotagede, Yogyakarta, Indonesia. 55172

                                      Phone. 0274-379772

05.Office address         : Faculty of Pharmacy, Gadjah MadaUniversity, Sekip Utara,

  Yogyakarta, Indonesia. 55281

  Phone 0274-542907, Fax 0274-543120

  e-mail: jadi_farma@ugm.ac.id and jadifarma@yahoo.com

 

08. Education

Education level

Place

Year

Title

Field

S-1

Profecy

S-2

S-3

UGM, Yogyakarta

UGM, Yogyakarta

ITB, Bandung

Dep. of Biochemistry Univ. of Leicester UK

1974

1976

1984

1991

Drs.

Parm.

MSc

PhD

Pharmacy

Pharmacy

Chemistry

Molecular Biology

 

09.Thesis

Topic               : Analysis of cloned genes for GABA catabolism

 

10. Main administrative duties:

Head,  Laboratory of Pharmaceutical Analysis, 2007 - Now

Secretary,  Faculty of Pharmacy Senate, 2005 - Now

Director, Biotechnology Research Center, 2004 – 2006

 

11. Area of Teaching:

Molecular Biology, Genetic Engineering, and Pharmaceutical Biotechnology

 

12. Research activities/interest:

Ribosome-inactivating proteins (RIPs) isolated from Mirabillis jalapa L for anticancer : acid and basic forms

 

13. Books

Topic

Publisher

Year

 

Structure elucidation

Separation methods

Food and Drug Analysis

Health Biotechnology

Quantitative Drug Analysis

 

 

Ghalia Indonesia

Kanisius

Pustaka Pelajar

Kanisius

UGM Press

 

 

1985

1988

2004

2008

2008

 

14. Publications:

                                                                         

Sudjadi, Sismindari, Herawati,T., Prasetyowati,A.T., 2003, Purification of Ribome-inactivating Protein (RIP) of Mirabililis jalapa L leaves by  CM-Sepharose CL-6B and Sephacryl S-300HR Columns, Indon.J.Pharm., 14 (2), 316-326

 

Sudjadi,  Ikawati.Z., Sismindari dan  Rahayu.P.R.S, 2004, Effects of pH, temperature and storage on the stability of MJ-30 protein isolated from Mirabilis jalapa L leaves, Indon.J.Pharm, 15: 44-49.

 

Sudjadi, 2004, Purification of Ribosome Inactivating Protein (RIP) from Annona squamosa L seeds by CM-Sepharose CL-6B column, Biology. 3(3): 181-190

 

Zullies Ikawati, Sudjadi, Widyaningsih Elly, Dyah Puspitasari, and Sismindari, 2003, Induction of apoptosis by protein fraction isolated from the leaves of Mirabilis jalapa L on HeLa and Raji cell-line, OPEM, 3(3): 151 – 156.

 

 Zullies Ikawati, Sudjadi and Sismindari, 2006,  Cytotoxicity against tumor cell-lines of a Ribosome-inativating Protein (RIP)-like protein isolated from leaves of Mirabilis jalapa L., Malaysian J Parm. Sci., 4(1): 1 - 12

 

Sudjadi, Lucia D Witasari, Modesta T Sadarum, Nia Nastity, and Sismindari, 2007, Cytotoxic effects of an acidic Ribosome-inactivating Protein Isolated from Mirabilis jalapa L leaves on cancer cell-lines,    Indon. J. Pharm, 18(1): 8 – 14.

 

Sudjadi and Sismindari, 2008, Recent progress in Ribosome-inactivating proteins (RIPs) for anticancer, Review, J. Trad.Med., in press.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

10. Researches

Topic

Sponsorship

Period

Transformation of the SMV coat protein gene to produce tolerant soy bean from virus infection

 

Applications of the DNA fingerprinting in Javanesse trait

 

Applications of recombinant DNA to produce transgenic chicken able to synthesis lisine

 

Production of high lysine silage using genetically engeenered Lactobacillus plantarum

 

Effects of Ribosome-Inactivating Protein from Annona squamosa L as antitumor

RUT I

 

 

DIKTI

 

RUT III

 

 

Competitive

Loan

 

RUT V

 

QUE-project,

 

QUE-project

1993-1995

 

 

1994

 

1994-1997

 

 

1997

 

 

1996-1999

 

2001-2002

 

2002-2003

 

11. Publications

 

Cloning of the SMV coat protein gene, Journal of Indonesian Plant Protection, 1995.

 

Cloning of the Escherichia coli K-12 dapD gene via PCR, Journal of Indonesian Biotechnology, 1997.

 

Overexpression and purification of SSA dehydrogenase and GABA transaminase from Escherichia coli K-10, Indonesian Journal of Pharmaciy, 1998.

 

Cloning of the Bacillus thuringensis cry gene , Journal of Indonesian Plant Protection, 1998

 

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